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991.
E M Sankila R Tolvanen J A van den Hurk F P Cremers A de la Chapelle 《Nature genetics》1992,1(2):109-113
Choroideremia (CHM) is an X-linked progressive degeneration of the choroid and retina. 12% of unrelated male patients carry deletions of the partially cloned CHM gene. In Finland, there are more than 120 living CHM patients belonging to eight apparently unrelated pedigrees. Molecular deletions involving the CHM gene have been detected in three families. We have screened the remaining five families for point mutations. In one large family a single nucleotide (T) insertion into the donor splice site of exon C leads to two aberrantly spliced mRNAs both producing a premature stop codon. The mutation can be assayed easily by amplification and digestion with Msel. Our findings provide additional evidence for the pathogenetic role of CHM mutations and provide a diagnostic tool for one fifth of the world's known CHM patients. 相似文献
992.
G. A. Schuiling N. Pols-Valkhof G. C. J. van der Schaaf-Verdonk F. H. de Jong T. R. Koiter 《Cellular and molecular life sciences : CMLS》1987,43(8):895-898
Summary The mid-pregnancy rescue of corpora lutea can be mimicked in the pseudopregnant rat by induction of decidual tissue in the uterus: in such rats, around day 10, there is neither luteolysis, nor resumption of follicle-development or increase of the pituitary responsiveness to LHRH. The results suggest that the mid-pregnancy rescue of corpora lutea is caused by a maternal factor.12 February 1987 相似文献
993.
994.
995.
Sequence similarity between the mammalian bmi-1 proto-oncogene and the Drosophila regulatory genes Psc and Su(z)2 总被引:20,自引:0,他引:20
The bmi-1 proto-oncogene can be activated by Moloney murine leukaemia proviral insertions in E mu-myc transgenic mice. It encodes a highly conserved nuclear protein of 324 amino acids which belongs to a family of proteins containing a putative new zinc-finger. Another closely related member of this family is the mouse protein Mel-18. Here we report on the cloning and characterization of a homologous gene (D-bmi) from Drosophila melanogaster. Our analysis indicates that distinct domains of the mouse Bmi-1 protein, including the putative zinc-finger motif, are highly conserved within the much larger D-Bmi protein. Chromosomal localization and sequence comparison reveal that D-bmi is identical to Posterior Sex Combs (Psc) and indicate that the conserved domains between mouse bmi and Psc are also conserved within Suppressor-2 of Zeste (Su(z)2). 相似文献
996.
Neuromuscular transmission in arterioles 总被引:2,自引:0,他引:2
997.
Stable nitroxides as lipid antioxidants 总被引:2,自引:0,他引:2
998.
Calcium channels in thrombin-activated human platelet membrane 总被引:6,自引:0,他引:6
Platelet-activating factor, 5-hydroxytryptamine, thromboxane A2, adenosine diphosphate and thrombin are known to activate platelets by stimulating calcium entry, but the nature of the entry pathways is unknown. We present the identification of single divalent cation channels from thrombin-activated human platelets. Membrane vesicles from unstimulated and thrombin-stimulated human platelets were incorporated in planar bilayers and unitary currents through single channels were measured. Divalent cation selective channels could only be demonstrated in thrombin-stimulated preparations. These channels share a number of properties in common with voltage-dependent calcium channels--a high degree of selectivity for divalent cations, a single channel conductance of about 10 pS (in 150 mM Ba2+) and sensitivity to blockade by inorganic calcium channel blockers such as Ni2+. In other respects, these channels are different as they are not voltage-dependent and are not blocked by 1,4-dihydropyridine calcium channel antagonists. 相似文献
999.
Wave-particle duality of C(60) molecules 总被引:6,自引:0,他引:6
Quantum superposition lies at the heart of quantum mechanics and gives rise to many of its paradoxes. Superposition of de Broglie matter waves' has been observed for massive particles such as electrons, atoms and dimers, small van der Waals clusters, and neutrons. But matter wave interferometry with larger objects has remained experimentally challenging, despite the development of powerful atom interferometric techniques for experiments in fundamental quantum mechanics, metrology and lithography. Here we report the observation of de Broglie wave interference of C(60) molecules by diffraction at a material absorption grating. This molecule is the most massive and complex object in which wave behaviour has been observed. Of particular interest is the fact that C(60) is almost a classical body, because of its many excited internal degrees of freedom and their possible couplings to the environment. Such couplings are essential for the appearance of decoherence, suggesting that interference experiments with large molecules should facilitate detailed studies of this process. 相似文献
1000.
Missing lithotroph identified as new planctomycete. 总被引:85,自引:0,他引:85
M Strous J A Fuerst E H Kramer S Logemann G Muyzer K T van de Pas-Schoonen R Webb J G Kuenen M S Jetten 《Nature》1999,400(6743):446-449
With the increased use of chemical fertilizers in agriculture, many densely populated countries face environmental problems associated with high ammonia emissions. The process of anaerobic ammonia oxidation ('anammox') is one of the most innovative technological advances in the removal of ammonia nitrogen from waste water. This new process combines ammonia and nitrite directly into dinitrogen gas. Until now, bacteria capable of anaerobically oxidizing ammonia had never been found and were known as "lithotrophs missing from nature". Here we report the discovery of this missing lithotroph and its identification as a new, autotrophic member of the order Planctomycetales, one of the major distinct divisions of the Bacteria. The new planctomycete grows extremely slowly, dividing only once every two weeks. At present, it cannot be cultivated by conventional microbiological techniques. The identification of this bacterium as the one responsible for anaerobic oxidation of ammonia makes an important contribution to the problem of unculturability. 相似文献